Jessie's Dustox is one of Jessie's Pokémon that she caught during her travels through Hoenn. Jessie caught Dustox on her way through Petalburg Woods as a Wurmple. It later evolved into a Cascoon and then a Dustox and became Jessie's main contest Pokémon during her Hoenn and Kanto contests. In Crossing Paths, Dustox was released during the Dustox Crossing to mate with a special colored Dustox and has not been seen since. Her Japanese voice actress is Chinami Nishimura, and her English voice actors are Amy Birnbaum (Seasons 6-8) and Michele Knotz (Seasons 9-111). As a Wurmple Tara Jayne voiced her, as a Cascoon Amy Birnbaum voiced her.
- Jessie's Dustox has something in common with Ash's Butterfree; both Pokemon were released by their trainers in order to have a relationship with another of their kind and also mated with their own species of a different color.